Annals of Colorectal Research Annals of Colorectal Research Ann Colorectal Res http://www.colorectalresearch.com 2322-5262 2322-5289 10.5812/acr. en jalali 2017 6 24 gregorian 2017 6 24 4 4
en 10.17795/acr-42856 Evaluating the Effect of Melillotus officinalis L. Aqueous Extracts on Healing of Acetic Acid-Induced Ulcerative Colitis in Male Rats Evaluating the Effect of <italic>Melillotus officinalis</italic> L. Aqueous Extracts on Healing of Acetic Acid-Induced Ulcerative Colitis in Male Rats research-article research-article Conclusions

This study revealed that M. officinalis had apparent curative effects on treating UC because of its antioxidant and anti-inflammatory activities.

Results

Treatment with M. officinalis aqueous extract could enhance colonic antioxidant capacity and decrease inflammation and acute colonic injury induced by acetic acid, which is dose-dependent. In addition, administrating the extract significantly (P ≤ 0.05) reduced the colonic level of malondialdehyde and myeloperoxidase, and significantly (P ≤ 0.05) increased the level of reduced glutathione (P ≤ 0.05). The extract had more effects at the dose of 2000 mg/kg than 1000 mg/kg dosage and prednisolone.

Methods

Fifty rats were randomly divided into five equal groups. Group I (Control healthy group) received 1 mL/kg of normal saline orally. Group II (control colitis group) received 1 mL/kg of normal saline orally. Group III (positive control) received 3 mg/kg prednisolone orally. Group IV received 1000 mg/kg M. officinalis aqueous extracts orally. Group V received 2000 mg/kg M. officinalis aqueous extracts orally. Ulcerative colitis was induced by intra-rectal acetic acid (3% v/v) administration. All treatments were done 24 hours after induction of colitis and continued for seven days. On the eighth day, the rats were sacrificed and colonic biopsies were taken for histopathological and biochemical studies. Data analysis was performed, using SPSS software and significance level was set at P ≤ 0.05.

Background

Ulcerative colitis (UC) is a form of inflammatory bowel disease (IBD) that is characterized by acute and chronic inflammation. The etiology and pathophysiology of IBD is unidentified, and there are many obstacles on the definite treatment of this disease. Recently, the useful effects of some herbal medicine on improving UC have been studied. Melillotus officinalis L. (M. officinalis) is an herb with antioxidant and anti-inflammatory effects used as food, forage and medicine.

Objectives

This study evaluated the antioxidant effects of M. officinalis aqueous extracts in the acetic acid- induced ulcerative colitis in rats.

Conclusions

This study revealed that M. officinalis had apparent curative effects on treating UC because of its antioxidant and anti-inflammatory activities.

Results

Treatment with M. officinalis aqueous extract could enhance colonic antioxidant capacity and decrease inflammation and acute colonic injury induced by acetic acid, which is dose-dependent. In addition, administrating the extract significantly (P ≤ 0.05) reduced the colonic level of malondialdehyde and myeloperoxidase, and significantly (P ≤ 0.05) increased the level of reduced glutathione (P ≤ 0.05). The extract had more effects at the dose of 2000 mg/kg than 1000 mg/kg dosage and prednisolone.

Methods

Fifty rats were randomly divided into five equal groups. Group I (Control healthy group) received 1 mL/kg of normal saline orally. Group II (control colitis group) received 1 mL/kg of normal saline orally. Group III (positive control) received 3 mg/kg prednisolone orally. Group IV received 1000 mg/kg M. officinalis aqueous extracts orally. Group V received 2000 mg/kg M. officinalis aqueous extracts orally. Ulcerative colitis was induced by intra-rectal acetic acid (3% v/v) administration. All treatments were done 24 hours after induction of colitis and continued for seven days. On the eighth day, the rats were sacrificed and colonic biopsies were taken for histopathological and biochemical studies. Data analysis was performed, using SPSS software and significance level was set at P ≤ 0.05.

Background

Ulcerative colitis (UC) is a form of inflammatory bowel disease (IBD) that is characterized by acute and chronic inflammation. The etiology and pathophysiology of IBD is unidentified, and there are many obstacles on the definite treatment of this disease. Recently, the useful effects of some herbal medicine on improving UC have been studied. Melillotus officinalis L. (M. officinalis) is an herb with antioxidant and anti-inflammatory effects used as food, forage and medicine.

Objectives

This study evaluated the antioxidant effects of M. officinalis aqueous extracts in the acetic acid- induced ulcerative colitis in rats.

Inflammatory Bowel Disease;Ulcerative Colitis;Antioxidant;Melillotus officinalis L Inflammatory Bowel Disease;Ulcerative Colitis;Antioxidant;Melillotus officinalis L http://www.colorectalresearch.com/index.php?page=article&article_id=42856 Nader Tanideh Nader Tanideh Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran Meghdad Bahrani Meghdad Bahrani Student Research Committee, Shiraz University of Medical Sciences, Shiraz, IR Iran Student Research Committee, Shiraz University of Medical Sciences, Shiraz, IR Iran Mohammad J. Khoshnood-Mansoorkhani Mohammad J. Khoshnood-Mansoorkhani Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, IR Iran Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, IR Iran Davood Mehrabani Davood Mehrabani Stem Cells and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran Stem Cells and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran Donya Firoozi Donya Firoozi School of Nutrition and Food Science, Shiraz University of Medical Sciences, Shiraz, IR Iran; School of Nutrition and Food Science, Shiraz University of Medical Sciences, Shiraz, IR Iran. Tel/Fax: +98-7137251007 School of Nutrition and Food Science, Shiraz University of Medical Sciences, Shiraz, IR Iran; School of Nutrition and Food Science, Shiraz University of Medical Sciences, Shiraz, IR Iran. Tel/Fax: +98-7137251007 Omid Koohi-Hosseinabadi Omid Koohi-Hosseinabadi Center of Experimental and Comparative Medicine, Shiraz University of Medical Sciences, Shiraz, IR Iran Center of Experimental and Comparative Medicine, Shiraz University of Medical Sciences, Shiraz, IR Iran Aida Iraji Aida Iraji Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz, IR Iran Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz, IR Iran