Annals of Colorectal Research

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Evaluating the Effect of Melillotus officinalis L. Aqueous Extracts on Healing of Acetic Acid-Induced Ulcerative Colitis in Male Rats

Nader Tanideh 1 , Meghdad Bahrani 2 , Mohammad J. Khoshnood-Mansoorkhani 3 , Davood Mehrabani 4 , Donya Firoozi 5 , * , Omid Koohi-Hosseinabadi 6 and Aida Iraji 7
Authors Information
1 Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran
2 Student Research Committee, Shiraz University of Medical Sciences, Shiraz, IR Iran
3 Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, IR Iran
4 Stem Cells and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran
5 School of Nutrition and Food Science, Shiraz University of Medical Sciences, Shiraz, IR Iran
6 Center of Experimental and Comparative Medicine, Shiraz University of Medical Sciences, Shiraz, IR Iran
7 Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz, IR Iran
Article information
  • Annals of Colorectal Research: December 01, 2016, 4 (4); e42856
  • Published Online: December 3, 2016
  • Article Type: Research Article
  • Received: October 10, 2016
  • Revised: November 23, 2016
  • Accepted: November 25, 2016
  • DOI: 10.17795/acr-42856

To Cite: Tanideh N, Bahrani M, Khoshnood-Mansoorkhani M J, Mehrabani D, Firoozi D, et al. Evaluating the Effect of Melillotus officinalis L. Aqueous Extracts on Healing of Acetic Acid-Induced Ulcerative Colitis in Male Rats, Ann Colorectal Res. 2016 ; 4(4):e42856. doi: 10.17795/acr-42856.

Copyright © 2016, Colorectal Research Center and Health Policy Research Center of Shiraz University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Jagtap AG, Shirke SS, Phadke AS. Effect of polyherbal formulation on experimental models of inflammatory bowel diseases. J Ethnopharmacol. 2004; 90(2-3): 195-204[DOI][PubMed]
  • 2. Papadakis KA, Targan SR. Role of cytokines in the pathogenesis of inflammatory bowel disease. Annu Rev Med. 2000; 51: 289-98[DOI][PubMed]
  • 3. Baumgart DC, Sandborn WJ. Inflammatory bowel disease: clinical aspects and established and evolving therapies. Lancet. 2007; 369(9573): 1641-57[DOI][PubMed]
  • 4. Loftus EJ. Clinical epidemiology of inflammatory bowel disease: Incidence, prevalence, and environmental influences. Gastroenterology. 2004; 126(6): 1504-17[PubMed]
  • 5. Aghazadeh R, Zali MR, Bahari A, Amin K, Ghahghaie F, Firouzi F. Inflammatory bowel disease in Iran: a review of 457 cases. J Gastroenterol Hepatol. 2005; 20(11): 1691-5[DOI][PubMed]
  • 6. Chen CY, Lee KT, Charles Tzu-Chi L, Lai WT, Huang YB. Epidemiology and Disease Burden of Ulcerative Colitis in Taiwan: A Nationwide Population-Based Study. Value Health Region Issues. 2013; 2(1): 127-34[DOI]
  • 7. Pavlick KP, Laroux FS, Fuseler J, Wolf RE, Gray L, Hoffman J, et al. Role of reactive metabolites of oxygen and nitrogen in inflammatory bowel disease. Free Radic Biol Med. 2002; 33(3): 311-22[PubMed]
  • 8. Mehrabani D, Ziaei M, Hosseini SV, Ghahramani L, Bananzadeh AM, Ashraf MJ, et al. The effect of calendula officinalis in therapy of acetic Acid induced ulcerative colitis in dog as an animal model. Iran Red Crescent Med J. 2011; 13(12): 884-90[PubMed]
  • 9. Mashtoub S, Hoang BV, Vu M, Lymn KA, Feinle-Bisset C, Howarth GS. Clinical and structural effects of traditional Chinese medicine and the herbal preparation, Iberogast, in a rat model of ulcerative colitis. J Evid Based Complementary Altern Med. 2014; 19(1): 10-9[DOI][PubMed]
  • 10. Dundar E, Olgun EG, Isiksoy S, Kurkcuoglu M, Baser KH, Bal C. The effects of intra-rectal and intra-peritoneal application of Origanum onites L. essential oil on 2,4,6-trinitrobenzenesulfonic acid-induced colitis in the rat. Exp Toxicol Pathol. 2008; 59(6): 399-408[DOI][PubMed]
  • 11. Mehrabani D, Bahrami F, Hosseini SV, Ashraf MJ, Tanideh N, Rezaianzadeh A, et al. The Healing Effect of Teucrium polium in Acetic Acid-Induced Ulcerative Colitis in the Dog as an Animal Model. Middle East J Dig Dis. 2012; 4(1): 40-7[PubMed]
  • 12. Mannasaheb BA, Kulkarni PV, Sangreskopp MA, Savant C, Mohan A. Protective effect of Agave americana Linn. leaf extract in acetic acid-induced ulcerative colitis in rats. Ayu. 2015; 36(1): 101-6[DOI][PubMed]
  • 13. Keshavarz A, Minaiyan M, Ghannadi A, Mahzouni P. Effects of Carum carvi L. (Caraway) extract and essential oil on TNBS-induced colitis in rats. Res Pharm Sci. 2013; 8(1): 1-8[PubMed]
  • 14. Al-Rejaie SS, Abuohashish HM, Al-Enazi MM, Al-Assaf AH, Parmar MY, Ahmed MM. Protective effect of naringenin on acetic acid-induced ulcerative colitis in rats. World J Gastroenterol. 2013; 19(34): 5633-44[DOI][PubMed]
  • 15. Mathew MM, Han NV, Murugesan A, Raj EA, Prasanth KG. Evaluation of the protective effect of Pterocarpus marsupium on acetic acid-induced ulcerative colitis in rats. Inflammopharmacology. 2015; 23(4): 195-201[DOI][PubMed]
  • 16. Anwer MS, Mohtasheem M, Azhar I, Ahmed SW, Bano H. Chemical constituents from Melilotus officinalis. J Basic Appl Sci. 2008; 4(2): 89-94
  • 17. Hirakawa T, Okawa M, Kinjo J, Nohara T. A new oleanene glucuronide obtained from the aerial parts of Melilotus officinalis. Chem Pharm Bull (Tokyo). 2000; 48(2): 286-7[PubMed]
  • 18. Hoult JR, Paya M. Pharmacological and biochemical actions of simple coumarins: natural products with therapeutic potential. Gen Pharmacol. 1996; 27(4): 713-22[PubMed]
  • 19. Plesca-Manea L, Parvu AE, Parvu M, Taamas M, Buia R, Puia M. Effects of Melilotus officinalis on acute inflammation. Phytother Res. 2002; 16(4): 316-9[DOI][PubMed]
  • 20. Eder M, Mehnert W. [The importance of concomitant compounds in plant extracts]. Pharmazie. 1998; 53(5): 285-93[PubMed]
  • 21. Born SL, Rodriguez PA, Eddy CL, Lehman-McKeeman LD. Synthesis and reactivity of coumarin 3,4-epoxide. Drug Metab Dispos. 1997; 25(11): 1318-24[PubMed]
  • 22. Weber US, Steffen B, Siegers CP. Antitumor-activities of coumarin, 7-hydroxy-coumarin and its glucuronide in several human tumor cell lines. Res Commun Mol Pathol Pharmacol. 1998; 99(2): 193-206[PubMed]
  • 23. Head KA, Jurenka JS. Inflammatory bowel disease part I: ulcerative colitis–pathophysiology and conventional and alternative treatment options. Alternat Med Rev. 2003; 8(3): 247-83
  • 24. Sharafati-Chaleshtori R, Rafieian-Kopaei M. Screening of antibacterial effect of the Scrophularia Striata against E. coli in vitro. J Herb Med Pharm. 2014; 3(1): 31-4
  • 25. Minaiyan M, Ghannadi A, Etemad M, Mahzouni P. A study of the effects of Cydonia oblonga Miller (Quince) on TNBS-induced ulcerative colitis in rats. Res Pharm Sci. 2012; 7(2): 103-10[PubMed]
  • 26. Tanideh N, Nematollahi SL, Hosseini SV, Hosseinzadeh M, Mehrabani D, Safarpour A, et al. The healing effect of Hypericum perforatum extract on acetic acid-induced ulcerative colitis in rat. Ann Colorect Res. 2014; 2(4)
  • 27. Subbarao KV, Richardson JS, Ang LC. Autopsy samples of Alzheimer's cortex show increased peroxidation in vitro. J Neurochem. 1990; 55(1): 342-5[PubMed]
  • 28. Mullane KM, Kraemer R, Smith B. Myeloperoxidase activity as a quantitative assessment of neutrophil infiltration into ischemic myocardium. J Pharmacol Methods. 1985; 14(3): 157-67[PubMed]
  • 29. Beutler E. Red cell metabolism: a manual of biochemical methods. 1975;
  • 30. Hartmann RM, Morgan Martins MI, Tieppo J, Fillmann HS, Marroni NP. Effect of Boswellia serrata on antioxidant status in an experimental model of colitis rats induced by acetic acid. Dig Dis Sci. 2012; 57(8): 2038-44[DOI][PubMed]
  • 31. Kruidenier L, Verspaget HW. Review article: oxidative stress as a pathogenic factor in inflammatory bowel disease--radicals or ridiculous? Aliment Pharmacol Ther. 2002; 16(12): 1997-2015[PubMed]
  • 32. Droge W. Free radicals in the physiological control of cell function. Physiol Rev. 2002; 82(1): 47-95[DOI][PubMed]
  • 33. Borges Bubols G, da Rocha Vianna D, Medina-Remon A, von Poser G, Maria Lamuela-Raventos R, Lucia Eifler-Lima V, et al. The Antioxidant Activity of Coumarins and Flavonoids. Mini Rev Med Chem. 2013; 13(3): 318-34[DOI]
  • 34. Trouillas P, Calliste C, Allais D, Simon A, Marfak A, Delage C, et al. Antioxidant, anti-inflammatory and antiproliferative properties of sixteen water plant extracts used in the Limousin countryside as herbal teas. Food Chemistry. 2003; 80(3): 399-407[DOI]
  • 35. Oetari S, Sudibyo M, Commandeur JN, Samhoedi R, Vermeulen NP. Effects of curcumin on cytochrome P450 and glutathione S-transferase activities in rat liver. Biochem Pharm. 1996; 51(1): 39-45[PubMed]
  • 36. Varanda EA, Raddi MS, Dias Fde L, Araujo MC, Gibran SC, Takahashi CS, et al. Mutagenic and cytotoxic activity of an isocoumarin (Paepalantine) isolated from Paepalanthus vellozioides. Teratog Carcinog Mutagen. 1997; 17(2): 85-95[PubMed]
  • 37. Gu J, Walker VE, Lipinskas TW, Walker DM, Ding X. Intraperitoneal administration of coumarin causes tissue-selective depletion of cytochromes P450 and cytotoxicity in the olfactory mucosa. Toxicol Appl Pharmacol. 1997; 146(1): 134-43[DOI][PubMed]
  • 38. Safarpour AR, Kaviyani F, Sepehrimanesh M, Ahmadi N, Hosseinabadi OK, Tanideh N, et al. Antioxidant and Anti-Inflammatory Effects of Gel and Aqueous Extract of Melilotus officinalis L. in Induced Ulcerative Colitis: A Rattus norvegicus Model. Ann Colorect Res. 2015; 3(2)
  • 39. Cetinkaya A, Bulbuloglu E, Kurutas EB, Ciralik H, Kantarceken B, Buyukbese MA. Beneficial effects of N-acetylcysteine on acetic acid-induced colitis in rats. Tohoku J Exp Med. 2005; 206(2): 131-9[PubMed]
  • 40. Cetinkaya A, Bulbuloglu E, Kantarceken B, Ciralik H, Kurutas EB, Buyukbese MA, et al. Effects of L-carnitine on oxidant/antioxidant status in acetic acid-induced colitis. Dig Dis Sci. 2006; 51(3): 488-94[DOI][PubMed]
  • 41. Hagar HH, El-Medany A, El-Eter E, Arafa M. Ameliorative effect of pyrrolidinedithiocarbamate on acetic acid-induced colitis in rats. Eur J Pharmacol. 2007; 554(1): 69-77[DOI][PubMed]
  • 42. Sivaprasad R, Nagaraj M, Varalakshmi P. Combined efficacies of lipoic acid and 2,3-dimercaptosuccinic acid against lead-induced lipid peroxidation in rat liver. J Nutr Biochem. 2004; 15(1): 18-23[PubMed]
  • 43. Sivaprasad R, Nagaraj M, Varalakshmi P. Lipoic acid in combination with a chelator ameliorates lead-induced peroxidative damages in rat kidney. Arch Toxicol. 2002; 76(8): 437-41[DOI][PubMed]
  • 44. Gonzalez R, Rodriguez S, Romay C, Gonzalez A, Armesto J, Remirez D, et al. Anti-Inflammatory Activity of Phycocyanin Extract in Acetic Acid-Induced Colitis in Rats. Pharmacol Res. 1999; 39(1): 1055-9[DOI][PubMed]
  • 45. Ukil A, Maity S, Karmakar S, Datta N, Vedasiromoni JR, Das PK. Curcumin, the major component of food flavour turmeric, reduces mucosal injury in trinitrobenzene sulphonic acid-induced colitis. Br J Pharmacol. 2003; 139(2): 209-18[DOI][PubMed]
  • 46. Sanchez-Fidalgo S, Sanchez de Ibarguen L, Cardeno A, Alarcon de la Lastra C. Influence of extra virgin olive oil diet enriched with hydroxytyrosol in a chronic DSS colitis model. Eur J Nutr. 2012; 51(4): 497-506[DOI][PubMed]
  • 47. Li XL, Cai YQ, Qin H, Wu YJ. Therapeutic effect and mechanism of proanthocyanidins from grape seeds in rats with TNBS-induced ulcerative colitis. Can J Physiol Pharmacol. 2008; 86(12): 841-9[DOI][PubMed]
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