Annals of Colorectal Research

Published by: Kowsar

Real Time PCR for Characterization of Enteroinvasive E. coli (EIEC) in Children with Diarrhea in Shiraz

Pejman Abbasi 1 , Mohammad Kargar 2 , * , Abbas Doosti 3 , Jalal Mardaneh 1 , Sadegh Ghorbani Dalini 2 and Mohammad Ali Dehyadegari 1
Authors Information
1 Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran
2 Department of Microbiology,Jahrom Branch Islamic Azad University, Jahrom, IR Iran
3 Biotechnology Research Center,Jahrom Branch Islamic Azad University, Shahrekord, IR Iran
Article information
  • Annals of Colorectal Research: September 1, 2014, 2 (3); e22721
  • Published Online: September 30, 2014
  • Article Type: Research Article
  • Received: August 14, 2014
  • Revised: October 5, 2014
  • Accepted: November 11, 2014
  • DOI: 10.17795/acr-22721

To Cite: Abbasi P, Kargar M, Doosti A, Mardaneh J, Ghorbani Dalini S, et al. Real Time PCR for Characterization of Enteroinvasive E. coli (EIEC) in Children with Diarrhea in Shiraz, Ann Colorectal Res. 2014 ; 2(3):e22721. doi: 10.17795/acr-22721.

Copyright © 2014, Colorectal Research Center and Health Policy Research Center of Shiraz University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
  • 1. Estrada-Garcia T, Lopez-Saucedo C, Thompson-Bonilla R, Abonce M, Lopez-Hernandez D, Santos JI, et al. Association of diarrheagenic Escherichia coli Pathotypes with infection and diarrhea among Mexican children and association of atypical Enteropathogenic E. coli with acute diarrhea. J Clin Microbiol. 2009; 47(1): 93-8[DOI][PubMed]
  • 2. Heydari H, Shokrollahi MR, Movahedi Z. Frequency of type 1 fimbriae among E. coli subtypes isolated from patients with urinary and gastrointestinal tract infection. Life Sci J. 2013; 10(7)
  • 3. Kagambega A, Martikainen O, Siitonen A, Traore AS, Barro N, Haukka K. Prevalence of diarrheagenic Escherichia coli virulence genes in the feces of slaughtered cattle, chickens, and pigs in Burkina Faso. Microbiologyopen. 2012; 1(3): 276-84[DOI][PubMed]
  • 4. Kalantar E, Alikhani MY, Naseri MH, Torabi V. Antibiotic resistance patterns of STEC and ETEC strains: a study on frozen foods of animal origin and children with acute diarrhea. J Microbiol Infect Dis. 2013; 3(1): 31-5
  • 5. Hegde A, Ballal M, Shenoy S. Detection of diarrheagenic Escherichia coli by multiplex PCR. Indian J Med Microbiol. 2012; 30(3): 279-84[DOI][PubMed]
  • 6. Guion CE, Ochoa TJ, Walker CM, Barletta F, Cleary TG. Detection of diarrheagenic Escherichia coli by use of melting-curve analysis and real-time multiplex PCR. J Clin Microbiol. 2008; 46(5): 1752-7[DOI][PubMed]
  • 7. Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, et al. Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers. J Microbiol Methods. 2011; 86(1): 119-20[DOI][PubMed]
  • 8. Gomez-Duarte OG, Romero-Herazo YC, Paez-Canro CZ, Eslava-Schmalbach JH, Arzuza O. Enterotoxigenic Escherichia coli associated with childhood diarrhoea in Colombia, South America. J Infect Dev Ctries. 2013; 7(5): 372-81[DOI][PubMed]
  • 9. Shams S, Haghi-Ashtiani MT, Nasrollahi L, Shahsiah R, Monajemzadeh M, Tahbaz-Lahafi B, et al. Frequency of shiga toxin-producing genes of Escherichia coli isolated from diarrheic stools of Iranian children by PCR. Iran J Pediatr. 2013; 23(6): 637-42[PubMed]
  • 10. Amisano G, Fornasero S, Migliaretti G, Caramello S, Tarasco V, Savino F. Diarrheagenic Escherichia coli in acute gastroenteritis in infants in North-West Italy. New Microbiol. 2011; 34(1): 45-51[PubMed]
  • 11. Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin Microbiol Rev. 1998; 11(1): 142-201[PubMed]
  • 12. Pereira AL, Giugliano LG. Adhesion of Diarrheagenic Escherichia coli and Inhibition by Glycocompounds Engaged in the Mucosal Innate Immunity. Biology (Basel). 2013; 2(2): 810-31[DOI][PubMed]
  • 13. Fujioka M, Kasai K, Miura T, Sato T, Otomo Y. Rapid diagnostic method for the detection of diarrheagenic Escherichia coli by multiplex PCR. Jpn J Infect Dis. 2009; 62(6): 476-80[PubMed]
  • 14. Song T, Toma C, Nakasone N, Iwanaga M. Sensitive and rapid detection of Shigella and enteroinvasive Escherichia coli by a loop-mediated isothermal amplification method. FEMS Microbiol Lett. 2005; 243(1): 259-63[DOI][PubMed]
  • 15. Smith CJ, Osborn AM. Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology. FEMS Microbiol Ecol. 2009; 67(1): 6-20[DOI][PubMed]
  • 16. Javadzadeh M, Dabiri S, Zangiabadi M. Role of Shigella, Enteroinvasive Escherichia coli (EIEC) and Entamoeba Histolytica in causing dysentry in children and antibiotic sensitivity testing. J Mazandaran Univ Med Sci. 2003; 39(13): 29-35
  • 17. Vieira N, Bates SJ, Solberg OD, Ponce K, Howsmon R, Cevallos W, et al. High prevalence of enteroinvasive Escherichia coli isolated in a remote region of northern coastal Ecuador. Am J Trop Med Hyg. 2007; 76(3): 528-33[PubMed]
  • 18. Hidalgo M, Realpe ME, Munoz N, Sicard D, Silva E, Agudelo CI, et al. [Acute diarrhea outbreak caused by Shigella flexneri at a school in Madrid, Cundinamarca: phenotypic and genotypic characterization of the isolates]. Biomedica. 2002; 22(3): 272-9[PubMed]
  • 19. Munoz N, Realpe ME, Castaneda E, Agudelo CI. [Characterization by pulsed-field gel electrophoresis of Salmonella typhimurium isolates recovered in the acute diarrheal disease surveillance program in Colombia, 1997-2004]. Biomedica. 2006; 26(3): 397-407[PubMed]
  • 20. Akbari A, Pourmand MR, Sharifi Yazdi MK, Soltan Dallal MM. Isolation of Enteroinvasive E. coli (EIEC) Strains In Children With Diarrhea By polymerase chain reaction (PCR) assay. J Fac Tehran Univ Med Sci. 2009; 3(3,4): 25-3
  • 21. Kagkli DM, Folloni S, Barbau-Piednoir E, Van den Eede G, Van den Bulcke M. Towards a pathogenic Escherichia coli detection platform using multiplex SYBR(R)Green Real-time PCR methods and high resolution melting analysis. PLoS One. 2012; 7(6)[DOI][PubMed]
  • 22. Souza TB, Lozer DM, Kitagawa SM, Spano LC, Silva NP, Scaletsky IC. Real-time multiplex PCR assay and melting curve analysis for identifying diarrheagenic Escherichia coli. J Clin Microbiol. 2013; 51(3): 1031-3[DOI][PubMed]
  • 23. Alikhani MY, Hashemi SH, Aslani MM, Farajnia S. Prevalence and antibiotic resistance patterns of diarrheagenic Escherichia coli isolated from adolescents and adults in Hamedan, Western Iran. Iran J Microbiol. 2013; 5(1): 42-7[PubMed]
  • 24. Sang WK, Oundo V, Schnabel D. Prevalence and antibiotic resistance of bacterial pathogens isolated from childhood diarrhoea in four provinces of Kenya. J Infect Dev Ctries. 2012; 6(7): 572-8[PubMed]
  • 25. Jafari F, Hamidian M, Rezadehbashi M, Doyle M, Salmanzadeh-Ahrabi S, Derakhshan F, et al. Prevalence and antimicrobial resistance of diarrheagenic Escherichia coli and Shigella species associated with acute diarrhea in Tehran, Iran. Can J Infect Dis Med Microbiol. 2009; 20(3)-62[PubMed]
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